/* ========================= Biofargo Table SOP (Embedded) ========================== */ .biofargo-wrap .biofargo-table-wrap{ overflow-x:auto; -webkit-overflow-scrolling:touch; border:1px solid #eee; border-radius:10px; padding:6px; background:#fff; margin-top:15px; } .biofargo-wrap table.biofargo-table{ width:100%; border-collapse:collapse; font-size:14px; min-width:640px; background:#fff; } .biofargo-wrap table.biofargo-table th, .biofargo-wrap table.biofargo-table td{ border:1px solid #e9ecef; padding:15px; vertical-align:middle; white-space:normal; word-break:break-word; } .biofargo-wrap table.biofargo-table thead th{ background:#f2f2f2; color:#000; font-weight:700; text-align:left; } .biofargo-wrap table.biofargo-table tbody td:first-child{ background:#f8f9fa; font-weight:600; width:30%; } Description BioFargo 100X Penicillin-Streptomycin Solution (commonly known as "Pen-Strep") is a broad-spectrum antibiotic mixture designed to prevent bacterial contamination in cell culture. This ready-to-use solution contains 10,000 units/mL of Penicillin G and 10,000 μg/mL of Streptomycin sulfate in a 0.85% saline or citrate buffer.Penicillin acts by inhibiting bacterial cell wall synthesis (targeting Gram-positive bacteria), while Streptomycin inhibits protein synthesis by binding to the 30S ribosomal subunit (targeting both Gram-negative and Gram-positive bacteria). Our solution is sterile-filtered (0.22 μm) and rigorously tested to ensure optimal cell growth and viability without toxicity at the recommended working concentration. Specification Full name 100X Penicillin-Streptomycin Solution Description Penicillin-Streptomycin Liquid,this solution is ready to use for cell culture after sterile filtration. It must be diluted 100-fold before use. Applications Cell culture Component Penicillin 10 KU/ml；Streptomycin 10 mg/ml；NaCl 0.9% Features Broad-Spectrum Protection: Highly effective against a wide range of Gram-positive and Gram-negative bacterial contaminants. Cell Culture Tested: Validated for use with various primary cells and immortalized cell lines to ensure no adverse effects on morphology or proliferation. Ready-to-Use 100X Concentrate: Simply dilute 1:100 in your basal medium (e.g., DMEM, RPMI 1640) for a 1X final working concentration. Sterile & Pure: 0.22 μm membrane filtered; tested for sterility, endotoxin levels, and mycoplasma. Superior Stability: Formulated for long-term frozen storage with excellent lot-to-lot consistency. Applications Routine Cell Culture: Prevention of bacterial infections in standard laboratory cell maintenance. Primary Cell Isolation: Safeguard against contamination during the sensitive initial phases of primary culture. Large-Scale Bioprocessing: Maintain sterile conditions in expanded cell populations. Tissue Engineering: Protection of sensitive scaffolds and tissue constructs from microbial growth. Components Penicillin 10 KU/ml Streptomycin 10 mg/ml NaCl 0.9% Storage -20°C FAQ What is 100X Penicillin-Streptomycin Solution used for? 100X Pen-Strep (Penicillin 10,000 IU/mL + Streptomycin 10 mg/mL) is used to prevent bacterial contamination in mammalian and primary cell culture. It is a broad-spectrum antibiotic mixture effective against both Gram-positive and Gram-negative bacteria. How do I prepare the working concentration from 100X stock? Dilute the 100X Penicillin-Streptomycin stock 1:100 in your basal cell culture medium (e.g., DMEM, RPMI 1640) to obtain a 1X working concentration suitable for routine use. Is this solution sterile and safe for sensitive cells? Yes. Biofargo Pen-Strep solution is sterile-filtered through a 0.22 μm membrane and validated for use with primary cells and immortalized cell lines. It does not adversely affect cell morphology or proliferation at recommended working concentrations. Which types of cells or applications are suitable for Pen-Strep? This solution is suitable for routine cell culture, primary cell isolation, tissue engineering, and large-scale bioprocessing where bacterial contamination needs to be prevented. It protects sensitive scaffolds and tissue constructs in vitro. What is the composition of Biofargo 100X Pen-Strep? Each mL contains Penicillin 10,000 IU, Streptomycin 10 mg, and 0.9% NaCl. Ready-to-use after dilution, sterile, and tested for endotoxin and mycoplasma. How should I store the 100X Pen-Strep stock? Store at -20 °C. Avoid repeated freeze-thaw cycles. Ensure sterility by using aseptic techniques when aliquoting for use. Can I use Pen-Strep with serum-free or chemically defined media? Yes. Biofargo 100X Pen-Strep is compatible with standard serum-containing, serum-free, and chemically defined cell culture media. Always dilute to 1X before use. Does Pen-Strep affect experimental outcomes? When used at the recommended 1X concentration, Pen-Strep effectively prevents bacterial contamination without interfering with cell growth, differentiation, or assay readouts. Excessive concentrations may affect sensitive cell types.
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