2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff™ HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff™ HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments. Features Convenient, ready-to-use mix Thermostable—half-life is more than 40 min at 95°C Generates PCR products with 3'-dA overhangs Applications Mouse genotyping Hotstart PCR Specifications Fidelity (vs. Taq) 1 × Hot Start No Overhang 3'-A Polymerase Taq DNA Polymerase Reaction Format SuperMix or Master Mix Reaction Speed Standard Product Type PCR Master Mix (2×) Components Name 10108ES03 10108ES08 10108ES50 10108ES60 2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye) 1 mL 5×1 mL 50×1 mL 100×1 mL Figure Cited from" Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9, Front. Oncol. , 23 June 2022. Molecular and Cellular Oncology, Volume 12 - 2022" Shipping and Storage Shipped on ice packs. Store at -20°C with a shelf life of 2 years. PCR Reaction（50 μL） Components Vol. Conc. ddH2O to 50 μL - DNA / - Primer F (10 μM) 2 μL 0.4 μM Primer R (10 μM) 2 μL 0.4 μM 2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) 25 μL 1× DNA Type Input Genomic DNA 50 ng-100 ng Plasmids 100 pg-20 ng cDNA 1-5 μL PCR Cycling Temp. Time Cycles 95°C 5 min 1 95°C 30 sec 35 50-60°C 30 sec 72°C 30 sec/kb 72°C 10 min 1 Customer Feedback 1.From Boston Children's Hospital: Genotyping performance significantly outperforms the previously used brand. Citations & References: [1] Han P, Cao P, Yue J, et al. Knockdown of hnRNPA1 Promotes NSCLC Metastasis and EMT by Regulating Alternative Splicing of LAS1L exon 9. Front Oncol. 2022;12:837248. Published 2022 Jun 23. doi:10.3389/fonc.2022.837248(IF:5.738) Documents: Safety Data Sheet 10108_MSDS_HB20251208 Manuals 10108_Manual_HB20251208
