ATP, Adenosine-5'-triphosphate, and its sodium salt are the basic components of energy change in many biological systems and can be used in a variety of molecular biology applications, such as kinase assays, in vitro transcription, ligation and phosphorylation reactions, etc. This product is a transparent colorless aqueous solution prepared from ATP trisodium salt, and free from DNase, RNase and phosphatase contamination.This product is produced in accordance with GMP process requirements and provided in liquid form. Feature Validated, product-specific process and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production Nucleotide in the multiple salt form（Na+, Tris etc) always available to meet different downstream application needs Application RNA synthesis and amplification Building block for in vitro transcription Specification CAS No 56-65-5 (free acid) Formula C10H13N5Na3O13P3 Molecular Weight 573.13 g/moL Purity(HPLC) ≥ 99% Content 100 mM ± 3 mM Structure Component Components No. Name 10129ES03 10129ES10 10129ES60 10129 ATP Solution GMP-grade (100 mM) 1 mL 10 mL 100 mL Storage The product should be stored at -25℃ ~ -15℃ for two years. Figures Standard RNA Synthesis Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1. Capped RNA Synthesis Figure 2. Synthesis of capped RNA in vitro. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B. Documents: Safety Data Sheet 10129_MSDS_HB220701 Manuals 10129_Manual_HB20260625
