GTP, Guanosine-5'-triphosphate, can be used in various of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. In addition, GTP plays an important role in signal transduction as a phosphate or pyrophosphate messenger: GTP can activate G proteins, which can induce multiple protein kinase mediated cascade reations, causing a variety of cytological behaviors, such as cell proliferation, differentiation, etc. GTP can also be used as a high-energy precursor of single nucleotides to participate in the synthesis of DNase and RNase. This product is a transparent colorless aqueous solution prepared with GTP tris solution, and free of DNase and RNase contamination. This product is produced in accordance with GMP process requirements and provided in liquid form. Feature Validated, product-specific process and analytical methods Product-specific stability Documentation follows applicable GMP guidelines AOF production process and raw materials (TSE & BSE) Nitrosamine statement Regulatory support documents available Large-scale production Nucleotide in the multiple salt form（Na+, Tris etc) always available to meet different downstream application needs Application RNA synthesis and amplification Building block for in vitro transcription Specification CAS No 86-01-1 (free acid); 36051-31-7 (3Na salt) Formula C10H13N5Na3O14P3 (free acid) Molecular Weight 589.13 g/moL (free acid) Purity (HPLC) ≥ 99% Content 100 mM ± 3 mM Structure Component Components No. Name 10132ES03 10132ES10 10132ES60 10132 CTP Solution GMP-grade (100 mM) 1 mL 10 mL 100 mL Shipping and Storage The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years. Figures Standard RNA Synthesis Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1. Capped RNA Synthesis Figure 2. Synthesis of capped RNA in vitro. The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B. Documents: Safety Data Sheet 10132_MSDS_HB220701 Manuals 10132_Manual_HB20260625 Related blog： DNase I Applications
