Lyticase is a specialized enzyme preparation for hydrolyzing yeast cell walls, widely used in downstream applications such as genome research, yeast artificial chromosome (YAC) extraction, yeast transformation, protoplast preparation, yeast cell disruption, industrial fermentation, and yeast inactivation. This product is supplied in liquid form at a concentration of 10 U/μL, with a typical working concentration of 0.15 U/μL. The optimal concentration should be adjusted according to your specific application, yeast strain, and cell density. For genomic DNA or plasmid DNA extraction, please follow the recommended concentration in the corresponding kit protocol. Features Broad-Spectrum: Lyses a wide range of fungal cells for versatile applications. Stable & Reliable: Consistent enzyme activity with easy storage and transport. High-Efficiency: Rapid nucleic acid purification and high-quality protoplast generation. Components Name 10403ES81 10403ES88 10403ES92 Lyticase (10 U/μL) 1500 U 3000 U 10000 U Storage This Product should be stored at -25°C ~ -15°C. Valid for 2 years. Application Yeast cell wall digestion Protoplast preparation from fungi Yeast transformation and genetic manipulation Extraction of genomic DNA or plasmid DNA from yeast Preparation of high-quality fungal RNA Industrial fermentation and yeast inactivation Fungal biotechnology research Documents: Safety Data Sheet 10403_MSDS_HB250909_EN.PDF Manuals 10403_Manual_HB20260605 Citations & References: [1] Lu H, Zhu J, Zhang T, et al. A rapid multiplex nucleic acid detection system of airborne fungi by an integrated DNA release device and microfluidic chip. Talanta. 2022;246:123467. doi:10.1016/j.talanta.2022.123467(IF:6.057)[2] Liao X, Zhao J, Liang S, et al. Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastoris. Biotechnol Biofuels. 2019;12:38. Published 2019 Feb 21. doi:10.1186/s13068-019-1377-z(IF:5.452)
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