RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form. Feature Improved resistance to oxidation, compared to human/porcine RNase inhibitor Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR) Isolated from a recombinant source Tested for the absence of DNases and RNases Application RT-PCR cDNA synthesis In vitro transcription/translation Enzymatic RNA labeling reaction Other applications where the integrity of RNA is important Specification Source Recombinant E.coli with Murine RNase inhibitor gene Storage Buffer 20 mM Hepes KOH，150 mM KCl，8 mM DTT，0.5%（v/v）NP 40，0.5%（v/v）Tween20，50%（v/v）Glycerol，pH 7.5 ±0.2 (25℃) Unit Definition The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit Components Components No. Name 10621ES10 (10 KU) 10621ES20 (20 KU) 10621ES60 (100 KU) 10621ES99 (1 MU) 10621 Murine RNase inhibitor GMP-grade (40 U/μL) 250 μL 500 μL 2.5 mL 25 mL Shipping and Storage The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year. Documents: Safety Data Sheet 10621_MSDS_HB260713 Manuals 10621_Manual_HB221111 Citations & References: [1] Shen W, Wang R, Fan Q, Li Y, Cheng Y. Natural polyphenol assisted delivery of single-strand oligonucleotides by cationic polymers. Gene Ther. 2020;27(7-8):383-391. doi:10.1038/s41434-020-0151-y(IF:4.128) Related blog： DNase I Applications
