This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates. Note: G* is the first base of the RNA transcript. The original brand name of the product is CleaScrip™. Feature Reduced dsRNA Content: dsRNA content is reduced by more than 10-fold. High Yield: Stable yield of over 9 mg/mL. Low Cap Analog Input**: Cap Analog input can be reduced to 2.5 mM. High Capping Efficiency**: Capping efficiency exceeds 99%. Please find information on the development of this enzyme. Components Components No. Name 10628ES10(10 KU) 10628ES60(100 KU) 10628ES72(250 KU) 10628ES86(2,500 KU) 10628 Hieff T7 RNA Polymerase (low dsRNA, 250 U/μL) 40 μL 400 μL 1 mL 10 mL [Note]: T7 RNA Polymerase can be used in combination with 10× Transcription Buffer 3 (Cat#10674). Specifications Source Recombinant E. coli with T7 RNA Polymerase gene Optimum Temperature 37℃ Storage Buffer 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25℃ Unit Definition The amount of enzyme required to incorporate 1 nmol of [3H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit. Recommended Mg2+ 30mM Magnesium Acetate QC Standard Items Specification/Standard Enzyme activity 250-300U/μL Protein Purity ≥95% Endotoxin
