Hieff Unicon Hotstart Direct Taq DNA Polymerase is a hotstart DNA polymerase that is resistant to blood and other inhibitors. The product is blocked by antibodies and has good amplification sensitivity and specificity. After heating at the pre-denaturation temperature, the antibody is completely inactivated and the DNA polymerase activity is released. The amplification induced by nonspecific annealing of primers could be effectively inhibited by using the hotstart Taq enzyme. Components Components No. Name 10718ES00 (500 U) 10718ES01 (5 KU) 10718ES94 (50 KU) 10718ES95 (1250 KU) 10718 Hotstart D-Taq (50 U/μL) 10 μL 100 μL 1 mL 25 mL Storage The product should be stored at -20°C for 2 years. Instructions 1. Reaction Composition Components Volume (μL) Final Concentration 2 × Buffera 25 1 × Primer/Probe Mixb X 0.1 -0.5 μM Hotstart D-Taq (50 U/μL)c 0.12 0.12 U/μL Template DNAb X 0.1-100 ng ddH2O to 50 - Note：Be sure to mix well before use, avoid excessive bubbles caused by violent vibration. a) According to the specific experimental application, it is needed to prepare the corresponding reaction buffer. If a basic buffer (10×) is required, Cat#11374 is recommended. b) The amount of DNA and the concentration of probes or primers are recommended concentrations. The optimal concentration can be adjusted according to the specific experimental conditions. c) The amount of enzyme can be adjusted according to the experimental application. 2. Optimized Cycling Protocol Reaction stage Temperature Time Cycle 1 Initial denaturation 95°C 5 min 1 2 Amplification reaction 95°C 15 sec 45 cycles 60°Ca 30 secb Note： a) Amplification reaction: The temperature is adjusted according to the Tm value of the designed primers. b) Fluorescence signal acquisition: Please set the experimental procedure according to the requirements of the instrument manual.
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