Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit is developed based on the principle of a next-generation topoisomerase that enables rapid and efficient ligation of DNA fragments. Coupled with a highly efficient blunt-ending factor, this kit supports fast cloning of both blunt-ended and sticky-ended PCR products. Features Fast: Ligation completes in just 5 minutes. Highly Efficient: Virtually no self-ligation; near 100% positive clone rate—no blue-white screening needed. Broad Insert Range: Supports fragments from 150 bp to 5 kb. Simple Workflow: Just add your purified insert—no extra steps required. Versatile Compatibility: Enhanced with blunt-end binding factor for seamless TA cloning and blunt-end cloning. Components Components No. Name 10906ES08 (5T) 10906ES20 (20 T) 10906ES50 (50 T) 10906-A 2× Hieff Clone™ Universal TOPO Cloning Premix 25 μL 100 μL 250 μL 10906-B 1 kb control insert (40 ng/μL) 10 μL 10 μL 10 μL 10906-C M13 Forward Primer(10 μM) 50 μL 200 μL 500 μL 10906-D M13 Reverse Primer(10 μM) 50 μL 200 μL 500 μL Storage This product should be stored at room temperature for 1 year. Application Molecular Cloning; Topo Cloning; Rapid Cloning Figures 1. Versatile Compatibility: TA cloning and blunt-end cloning. Figure 1. High cloning efficiency of the Hieff Clone™ Universal Zero TOPO TA/Blunt Cloning Kit. With 10 ng of a 1 kb insert (either blunt- or sticky-ended), the kit yields abundant colonies and achieves 100% positive clone rate. 2. Stong Stability (A).Accelerated Stability (B).Freeze-Thaw Stability Figure 2. Stability Assessment (A).Accelerated Stability: The Hieff Clone® Universal Zero TOPO TA/Blunt Cloning Kit remains stable after 7 days at 37°C. (B).Freeze-Thaw Stability: The kit maintains performance after 50 freeze-thaw cycles. Documents: Safety Data Sheet 10906_MSDS_HB251120_EN.PDF Manuals 10906_Manual_Ver.EN20251120.pdf
