Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl groups of two adjacent oligonucleotides hybridized to the same complementary target DNA strand. This catalytic reaction occurs only when the two oligonucleotides are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotides. Therefore, it can be used to detect single-base substitutions. Taq DNA Ligase uses NAD+ as a cofactor and is active in the temperature range of 45°C to 65°C. Applications Specific detection of alleles using ligase detection reaction and ligase chain reaction Mutagenesis by PCR amplification incorporating phosphorylated oligonucleotides Homologous recombination Specifications Concentration 40 U/μL Source Recombinant E. coli strain containing the ligase gene cloned from Thermus aquaticus HB8. Unit Definition One unit of activity is defined as the amount of enzyme required to ligate 50% of 1 µg of λDNA fragments digested with BstEII (12 bp sticky ends) within 15 minutes at 45°C in a 50 µL reaction volume Inactivation conditions Incubate at 45°C for 15 minutes. Add stop dye (50% glycerol, 50 mM EDTA, and bromophenol blue) to terminate the reaction Components Components No. Name 11051ES80 11051ES84 11051ES92 11051-A Taq DNA Ligase (40 U/µL) 25 μL 50 μL 5×50 μL 11051-B 10×Taq DNA Ligase Buffer（NAD+） 250 μL 500 μL 5×500 μL Shipping and Storage This product should be stored at -25 ~ -15℃ for 1 year. Documents: Safety Data Sheet 11051_MSDS_Ver.EN20250226.pdf Manuals 11051_Manual_Ver.EN20250226.pdf
