T4 UvsX Recombinase, derived from the T4 bacteriophage, is a homolog of the RecA/Rad51 family and plays a critical role in the repair of double-strand DNA breaks and the restart of replication forks. This recombinase collaborates with other DNA-binding proteins or cofactors to form a nucleoprotein complex with single-stranded DNA (ssDNA). The complex identifies complementary regions on target DNA for hybridization, facilitating subsequent strand displacement reactions. The enzyme exhibits no nuclease activity. Product Components NO. Name 11079ES60 (100 μg) 11079ES03 (1 mg) 11079ES08 (5 mg) 11079ES20 (20 mg) 11079 T4 UvsX protein (2 μg/μL) 50 μL 500 μL 2.5 mL 10 mL Product Applications Primarily used in isothermal amplification techniques, such as Recombinase Polymerase Amplification (RPA). Enzyme Storage Buffer Composition 50 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 20% Glycerol, pH 7.5 at 25°C. Shipping and Storage Conditions Shipped with ice packs; store at -20°C. Shelf life: 2 years. Avoid repeated freeze-thaw cycles. Precautions 1. The glycerol content in the T4 UvsX Recombinase storage buffer is 20%. It is recommended to aliquot the enzyme into separate portions to avoid repeated freeze-thaw cycles.2. This product is intended for research use only.3. For your safety and health, please wear a lab coat and disposable gloves during handling.
