The product is a premixed solution for 2× real-time quantitative PCR amplification by the probe method. The antibody hot-start UNICONTM DNA Polymerase used can effectively inhibit the amplification caused by the non-specific annealing of primers. At the same time, the formula optimizes the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of PCR reaction, which is suitable for the amplification of low-concentration templates, so that quantitative PCR can obtain a good standard curve in a wide quantitative area. Features High specificity High sensitivity Applications Gene Expression Specifications Hot Start Built-in hot start Detection method Primer-probe detection PCR method RT-qPCR Polymerase Taq DNA polymerase Type of sample RNA Application equipment Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne™ Plus; 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6, 7, 12k Flex; Bio-Rad：CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf：Mastercycler ep realplex, realplex 2 s; Qiagen：Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science：LightCycler 480, LightCycler 2.0; Lightcycle r 96; Thermo Scientific：PikoReal Cycler; Cepheid：SmartCycler; Illumina：Eco qPCR. Components Components No. Name 11205ES03 11205ES08 11205ES25 11205-A Hieff Unicon™ qPCR TaqMan Probe Master Mix 1 mL 5×1 mL 25 mL 11205-B 50×Low Rox 40 μL 200 μL 1 mL 11205-C 50×High Rox 40 μL 200 μL 1 mL Storage The product should be stored at -25°C~-15℃ away from light for 18 months. Please avoid repeated freeze-thaw. Documents: Safety Data Sheet 11205_MSDS_HB220420 Manuals 11205_Manual_Ver.HB230112 Citations & References: [1] Huang T, Li X, Wang F, et al. The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy. Cell Death Dis. 2021;12(4):333. Published 2021 Mar 29. doi:10.1038/s41419-021-03629-4(IF:8.469) [2] Ma H, Lin J, Li L, et al. Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis. Chemosphere. 2021;282:131149. doi:10.1016/j.chemosphere.2021.131149(IF:7.086) [3] Lu L, Li X, Zhong Z, et al. KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy. Int J Biol Sci. 2021;17(15):4093-4107. Published 2021 Oct 3. doi:10.7150/ijbs.62867(IF:6.582) [4] Lu L, Zhong Z, Gu J, Nan K, Zhu M, Miao C. ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy. Mol Med. 2021;27(1):74. Published 2021 Jul 8. doi:10.1186/s10020-021-00339-7(IF:6.354) [5] Li X, Lu L, Hou W, et al. The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy. J Transl Med. 2022;20(1):147. Published 2022 Mar 29. doi:10.1186/s12967-022-03352-4(IF:5.531) [6] Shen X, Chen X, Wang J, et al. SET8 suppression mediates high glucose-induced vascular endothelial inflammation via the upregulation of PTEN. Exp Mol Med. 2020;52(10):1715-1729. doi:10.1038/s12276-020-00509-3(IF:5.418) [7] Qi J, Chen X, Wu Q, et al. Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression. Oxid Med Cell Longev. 2020;2020:3985089. Published 2020 Mar 19. doi:10.1155/2020/3985089(IF:5.076) [8] Zeng W, Ren J, Li Z, et al. Levistolide A Inhibits PEDV Replication via Inducing ROS Generation. Viruses. 2022;14(2):258. Published 2022 Jan 27. doi:10.3390/v14020258(IF:5.048) [9] Li D, Ma H, Shu Q, et al. Arsenite inhibits M2a polarization of macrophages through downregulation of peroxisome proliferator-activated receptor gamma [published online ahead of print, 2022 Jun 28]. Toxicol Appl Pharmacol. 2022;450:116142. doi:10.1016/j.taap.2022.116142(IF:4.460) [10] Long J, Liu L, Zhou X, Lu X, Qin L. HLA-DQB1-AS1 Promotes Cell Proliferation, Inhibits Apoptosis, and Binds with ZRANB2 Protein in Hepatocellular Carcinoma. J Oncol. 2022;2022:7130634. Published 2022 May 11. doi:10.1155/2022/7130634(IF:4.375) [11] Hou W, Lu L, Li X, Sun M, Zhu M, Miao C. c-Myc participates in high glucose-mediated endothelial inflammation via upregulation of IRAK1 expression in diabetic nephropathy. Cell Signal. 2022;92:110263. doi:10.1016/j.cellsig.2022.110263(IF:4.315) [12] Ma H, Ding Z, Xie Y, et al. Methylglyoxal produced by tumor cells through formaldehyde-enhanced Warburg effect potentiated polarization of tumor-associated macrophages. Toxicol Appl Pharmacol. 2022;438:115910. doi:10.1016/j.taap.2022.115910(IF:4.219) [13] Cao N, Tang Z, Zhang X, et al. Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1. Front Vet Sci. 2022;8:792322. Published 2022 Feb 8. doi:10.3389/fvets.2021.792322(IF:3.412) [14] Qi J, Wu Q, Cheng Q, Chen X, Zhu M, Miao C. High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression. J Diabetes Res. 2020;2020:2308520. Published 2020 Feb 23. doi:10.1155/2020/2308520(IF:2.965) Related blog： What to do when the qPCR results are weird?
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