The product is a premixed solution for 2× real-time quantitative PCR amplification by the probe method. The antibody hot-start UNICON™ DNA Polymerase used can effectively inhibit the amplification caused by the non-specific annealing of primers. At the same time, the formula optimizes the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of PCR reaction, which is suitable for the amplification of low-concentration templates, so that quantitative PCR can obtain a good standard curve in a wide quantitative area. Features High specificity High sensitivity Applications Gene Expression Specifications Hot Start Built-in hot start Detection method Primer-probe detection PCR method RT-qPCR Polymerase Taq DNA polymerase Type of sample RNA Application equipment Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne™ Plus; 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6, 7, 12k Flex; Bio-Rad:CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf:Mastercycler ep realplex, realplex 2 s; Qiagen:Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science:LightCycler 480, LightCycler 2.0; Lightcycle r 96; Thermo Scientific:PikoReal Cycler; Cepheid:SmartCycler; Illumina:Eco qPCR. Components Components No. Name 11205ES03 11205ES08 11205ES25 11205-A Hieff Unicon™ qPCR TaqMan Probe Master Mix 1 mL 5×1 mL 25 mL 11205-B 50×Low Rox 40 μL 200 μL 1 mL 11205-C 50×High Rox 40 μL 200 μL 1 mL