Hifair™ III Reverse Transcriptase, 600 U/μL, Glycerol-free _ 11297ES

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Hifair™ III Reverse Transcriptase is a novel reverse transcriptase engineered from Hieff™ M-MLV (H-) Reverse Transcriptase. Compared to its predecessor, Hifair™ III exhibits signif icantly enhanced thermostability, tolerating reaction temperatures of up to 65°C, making it ideal for reverse transcribing RNA templates with complex secondary structures. Additionally, this enzyme features increased template affinity, making it highly suitable for reverse transcription of limited samples and low-copy genes. Its ability to synthesize full-length cDNA has also been improved, capable of extending cDNA up to 19.8 kb. This glycerol-free version (600 U/μL) is specifically designed for the preparation of lyophilized formulations and lyophilizable RT-LAMP reagents. Specifications Cat.No. 11297ES09 / 11297ES12 / 11297ES75 Size 6 KU / 12 KU / 300 KU Components Components No. Name 11297ES09 (6 KU) 11297ES12 (12 KU) 11297ES75 (300 KU) 11297 Hifair™ III Reverse Transcriptase (600 U/µL, Glycerol-free) 10 μL 20 μL 500 μL Storage This product should be stored at 2~8℃ for 6 months. Applications Preparation of lyophilized formulations Lyophilizable RT-LAMP kits Activity Definition One unit (U) is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid-insoluble material using Poly(A)·Oligo(dT) as the template-primer in 10 minutes at 37°C. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. 3. Maintain a clean experimental area. Wear clean gloves and a mask during operation. Ensure all consumables are RNase-free to prevent RNase contamination. 4. Perform all operations on ice to prevent RNA degradation. 5. High-quality RNA samples are recommended to ensure efficient reverse transcription. Instructions 1. Reaction System Setup Cat. No. Component Volume 13840-A 10×Bst Buffer 2.5 µL 13840-C 100 mmol/L MgSO4 2 µL dNTP Mix (100 mmol/L) dNTP Mix (100 mmol/L) 3.5 µL dUTP Mix (100 mmol/L)(Optional) dUTP Mix (100 mmol/L)（Optional） 0.35 µL 11297 Hifair™ III Reverse Transcriptase (600 U/µL, Glycerol-free) 0.1 µL 10703 Murine RNase inhibitor (200 U/µL, Glycerol-free) 0.2 µL 13839(Optional) Heat Labile UDG (200 U/µL)（Optional） 0.005 µL 10×Primersa 10×Primers 2 µL 13840-Bb Bst Ⅱ Plus DNA polymerase (120 U/µL, Glycerol-free) 0.5 µL RNase free ddH2O RNase free ddH2O To 25 µL [Note]: a. 10× Primers: 16 µmol/L FIP/BIP, 2 µmol/L F3/B3, 4 µmol/L Loop F/B each. The primer ratios can be adjusted as needed. b. Volumes can be adjusted according to specific experimental conditions. 2. Reaction Program Settings: Temperature Time Function 37℃ 2 min Digestion 65℃ 30-60 mina Reverse Transcription and Isothermal Amplification 85℃ 10 min Enzyme Inactivation [Note]: a. For probe-based methods, fluorescence can be collected directly in the target channel at 1-minute intervals. For dye-based methods, you may additionally add Dye (Cat# 13841), select the SYBR channel, and collect fluorescence at 1-minute intervals. Documents: Safety Data Sheet 11297_MSDS_HB260630 Manuals 11297_Manual_HB20260630 Show more