Hieff NGS™ EvoMax RNA Library Prep Kit（dNTP） _ 12341ES

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Hieff NGS™ EvoMax RNA Library Prep Kit (dNTP) is designed for total RNA sequencing library preparation and is compatible with both Illumina and MGI platforms. Available in two form ats—standard tube and pre-loaded plate—the plate format eliminates manual preparation and sub-packaging, enabling direct integration with automated library construction for greater convenience. The kit includes reagents for RNA fragmentation, reverse transcription, ds-cDNA synthesis, and library amplification. It can be seamlessly combined with mRNA purification or rRNA depletion kits. All components undergo rigorous quality control and functional validation to ensure stable, reproducible library preparation results. Features Premixed Reagents: Enzymes and buffers are pre-mixed to reduce reagent components and simplify preparation. Safe and light-stable: The non-specific reagent requires no additional light protection, ensuring safe handling and consistent, reliable results. Preloaded Plate Format—Ready to Use: Designed for seamless integration with automation instruments, the preloaded plates enable easy “tear-and-use” operation for convenient automated workflows. Components Components No. Name 12341ES08 12341ES24 12341ES96 12341ES97 (Tube Automation 96T) 12341ES98 (Plate Automation 96T) 12341-A Frag/Prime Buffer 150 μL 450 μL 2×900 μL 2×1064 μL 8×266 μL 12341-B 1st Reaction Module 2.0 64 μL 192 μL 768 μL 960 μL 8×120 μL 12341-C 2nd Reaction Module(dNTP) 280 μL 840 μL 3×1120 μL 3×1280 μL 8×480 μL 12341-D Ligation Reaction Module 280 μL 840 μL 3×1120 μL 3×1280 μL 8×480 μL 12341-E 2×Super Canace™ II High-Fidelity Mix 200 μL 600 μL 2×1200 μL 2×1360 μL 8×340 μL * Primer Mix* / / / / / [Note]: * The notation indicates that the component is not included in the kit. Primer mix is required if the complete adapters are used, otherwise it isn’t. This kit is compatible with Illumina and MGI platforms, but need additional primer mix (Cat # 13334 Primer Mix for MGI and Cat # 13335 Primer Mix for Illumina) for Illumina or MGI platform if the complete adapters are used. ** See the following diagram for the layout of plate reagent group. Storage This product should be stored at -25~-15℃ for one year.. Application RNA library Preparation; Transcriptome profiling and gene expression analysis; mRNA sequencing for coding RNA studies; lncRNA (long non-coding RNA) research; Small RNA sequencing (if applicable); Differential gene expression analysis; RNA biomarker discovery; RNA variant and fusion detection. Figures 1.Simplified Product Format Figure 1. Simplied components of EvoMax RNA Library Prep Kit. 2.Fast RNA Library preparation Figure 2. Premixed RNA Library Prep: More Efficient and Time-Saving 3.Automation-friendly Figure 3. Highly uniform mRNA and lncRNA libraries prepared with 96-channel automation. 4.Strong Stability Figure 4. Real-time stability: After 18 months of storage, performance remains comparable to the reference kit. Documents: Safety Data Sheet 12341_MSDS_HB250620_EN.PDF Manuals 12341_Manual_HB20260401.pdf Show more