DNA polymerase I (10 U/μL) _ 12903ES

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DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecula r weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity. Applications With DNase I (Cat#10607) for nick translation reaction With RNase H (Cat#12906) to synthesize the second strand of cDNA Specifications Concentration 10 U/μL Unit Definition One unit is defined as the amount of enzyme required to degrade 1 µg of circular plasmid within a 10-minute period at 37°C in a 100 µl reaction system Components Components No. Name 12903ES60 12903ES76 12903ES92 12903-A DNA polymerase I (10 U/μL) 50 μL 250 μL 1 mL 12903-B 10×DNA polymerase I Reaction Buffer 1 mL 1×5 mL 4×5 mL Shipping and Storage This product should be stored at -25 ~ -15℃ for 2 years. Documents: Safety Data Sheet 12903_MSDS_Ver.EN20241210.pdf Manuals 12903_Manual_Ver.EN20250729.pdf Related Blog DNase I Applications Show more