RNase H(5U/μL) _ 12906ES

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RNase H (ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds in RNA within DNA/RNA hybrids, but does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA or RNA. Yeasen offers comprehensive solutions for experiments. Explore our related products: Ultra-Clean UCF.ME™ Thermostable RNase H: Precise Cutting for More Efficient Experiments! Applications Removal of mRNA before the second-strand synthesis of cDNA Removal of RNA after the first-strand synthesis of cDNA in RT-PCR/RT-qPCR experiments Removal of poly(A) after hybridization of Oligo(dT) with mRNA Cleavage at specific sites in RNA Specifications Source Shigella flexneri Molecular Weight 19.7 KDa Concentration 5 U/μL Unit Definition One unit is defined as the the amount needed to hydrolyze RNA-DNA hybrids and produce 1 nmol of ribonucleotides in a 50 μL reaction system within 20 minutes at 37°C Components Components No. Name 12906ES60 12906ES76 12906ES90 12906-A RNase H(5 U/μL) 20 μL 100 μL 1 mL 12906-B 10×RNase H Reaction Buffer* 200 μL 1 mL 10×1 mL Note: *10×RNase H Reaction Buffer: 750 mM KCl, 500 mM Tris-HCl, 30 mM MgCl2, 100 mM Dithiothreitol, pH 8.3 @ 25℃. Shipping and Storage This product should be stored at -25 ~ -15℃ for 2 years. Documents: Safety Data Sheet 12906-COMPONENT A_MSDS_HB220428 12906-COMPONENT B_MSDS_HB220428 Manuals 12906_Manual_HB220412 Related Blog： DNase I Applications Show more