Hieff NGS™ DNA Library Prep Kit 2.0 (Mechanical) _ 12927ES

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Hieff NGS™ DNA Library Prep Kit is a new generation library preparation kit specially developed and designed for Illumina&MGI sequencing platform. On the basis of the previous gene ration of library preparation kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA. Features Broad Sample Compatibility: Supports a wide range of input DNA from 100 pg to 1 µg, covering all common sample types. High Adapter Ligation Efficiency: Powered by a novel ligase mutant to minimize adapter dimers and maximize library conversion rates. Accurate Mutation Detection: Incorporates a next-generation high-fidelity enzyme to enhance the precision of low-frequency variant detection. Excellent Reagent Stability: Single-use library prep reagents remain stable for up to 4 weeks at 4°C and 2 weeks at 25°C, fully compatible with automated workflows Components Components No. Name 12927ES08 (8 T) 12927ES24 (24 T) 12927ES96 (96 T) 12927ES97(Tube-basedAutomation) 12927ES99 12927-A Endprep Buffer 2.0 56 μL 168 μL 672 μL 960 μL 9600μL 12927-B Endprep Enzyme 2.0 24 μL 72 μL 288 μL 390 μL 3900μL 12927-C Ligation Enhancer 2.0 240 μL 720 μL 3×960 μL 3×1070 μL 32.1 mL 12927-D Rapid T4 DNA Ligase 2.0 80 μL 240 μL 2×480 μL 1210 μL 12.1 mL 12927-E Canace™ Pro Amplification Mix 200 μL 600 μL 3×800 μL 3×940 μL 28.2mL Shipping and Storage This product should be stored at -25~-15℃ for 1 year.. Application DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing; Figures 1. High Library Conversion Rate Figure 1. Using 170 bp DNA fragments to simulate cfDNA, Yeasen T4 DNA ligase demonstrated exceptional adapter ligation efficiency—up to 80%! 2. Multi-Species Compatibility Figure 2. Library Yield Comparison Across Species Library preparation was performed using 50 ng of genomic DNA from different species. Yeasen’s 12927ES (2G) kit produced equal or higher library yields than 13577ES (1G), and showed superior performance compared to supplier N* and K*. 3. Compatible with Illumina and MGI platforms Figure 3. Compatibility of UDI Adapters and complete adapter with Illumina and MGI Platforms 4. Excellent Sequencing Data Performance Figure 4. Comparison of Sequencing Data Quality Using tumor SNV and wild-type gDNA/ctDNA/FFPE DNA standards with 50 ng/10 ng input and 6-plex hybrid capture (Cancer SLC Panel), Kit 12927 showed lower mismatch, insertion, and deletion rates, indicating higher data accuracy. 5. Stability Figure 5. Stability evaluation of the library preparation kit under different storage conditions. Library yield remained consistent with the control after storage at 4 °C and 25 °C for 1 to 4 weeks, indicating good reagent stability under short-term elevated temperatures. 6. Higher stability of single-tube reagents, adaptable to automated workstations The single-tube reagent 12927ES showed no declining trend in library yield after being stored at 4°C for 4 weeks and 25°C for 2 weeks, demonstrating greater advantages for automation compatibility. 7. Suitable for library preparation with FFPE samples of varying qualities Using FFPE samples of different qualities as templates for library preparation, under the same cycle number, 12927ES exhibited a significant advantage in library yield compared to 13577ES, 12955ES, as well as equivalent products from Company A and Company B. 8. Suitable for metagenomic sample library preparation Using different soil samples as templates for library preparation, under the same cycle number, 12927ES showed a significant advantage in library yield compared to 13577ES, 12955ES, as well as equivalent products from Company A and Company B. 9. Lower false-positive mutation rate Using 100 ng of reference material as a template, WES hybrid capture sequencing comparative experiments were performed using 12927ES and other library preparation reagents. The results demonstrated that 12927ES outperformed other products in metrics such as Mismatch Ratio, Insertion Ratio, and Deletion Ratio. Documents: Safety Data Sheet 12927_MSDS_HB250620_EN.PDF Manuals 12927_Manual_HB20260402.pdf Show more