Hieff NGS™ Dual Barcode Fast-Pace DNA Cyclization Kit for MGI -13340ES

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The Hieff NGS™ Dual Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and op timized Buffer significantly improve reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard dual-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents. Features Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.) Fast cyclization: a 1-hour rapid cyclization High cyclization efficiency: the cfDNA cyclization efficiency>30%, other sample types>40% Applications To prepare a single-stranded circular DNA library dedicated to the MGI high-throughput sequencer Specifications Product Type NGS Library Preparation Product Line DNA library Preparation Input DNA The recommended Input DNA is 1 pmol, if the PCR product is insufficient, down to 0.5 pmol For Use With (Application) Dual Barcode DNA Cyclization Kit for MGI For Use With (Equipment) MGI Platforms Quantity 16/96 Reactions Components Components No. Name 13340ES16 (16T) 13340ES96 (96T) 13340-A DB Splint Oligo 96 μL 576 μL 13340-B Splint Buffer 240 μL 2×720 μL 13340-C Ligase 80 μL 480 μL 13340-D Digestion Buffer 128 μL 768 μL 13340-E Digestion Enzyme 32 μL 192 μL Storage -25℃ ~ -15℃ storage, valid for one year. Documents: Safety Data Sheet 13340-COMPONENT A-MSDS-HB220420.pdf 13340-COMPONENT B-MSDS-HB220420.pdf 13340-COMPONENT C-MSDS-HB220420.pdf 13340-COMPONENT D-MSDS-HB220420.pdf 13340-COMPONENT E-MSDS-HB220420.pdf Manuals 13340_Manual_HB230112_EN.pdf Show more