Hieff™ Super Bst DNA Polymerase(120 U/μL) _ 14411ES

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Hieff™ Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp. DNA Polymerase I and has been genetically engineered to eliminate its 5’→3’ exonuclease activity. This product exhibits strong 5’→3’ DNA polymerase activity, strand-displacement activity, and tolerance to dUTP, making it particularly suitable for contamination-controlled isothermal amplification reactions such as LAMP and CPA. Compared to Bst Plus DNA Polymerase, Hieff™ Super Bst DNA Polymerase demonstrates enhanced thermostability, which improves amplification performance. The enzyme is well-suited for low-template-concentration assays and high-temperature LAMP reactions (e.g., at 70°C), effectively enhancing detection specificity and shortening time-to-result. Its thermostable nature also enables the development of hot-start Bst formulations for further improvement in assay specificity. Specifications Cat NO. 14411ES03 / 14411ES05 / 14411ES08 Size 6,000 U / 24,000 U / 120,000 U Components Components No. Name 14411ES03 (6000 U) 14411ES05 (24000 U) 14411ES08 (120000 U) 14411-A Hieff™ Super Bst DNA Polymerase(120 U/μL) 50 μL 200 μL 1 mL 14411-B 10× Hieff™ Super Bst DNA Polymerase Buffer 1 mL 1 mL 3 × 1 mL 14411-C 100 mM MgSO4 1 mL 1 mL 3 × 1 mL Applications This product is suitable for various isothermal amplification methods, including LAMP, CPA, and RCA. It supports LAMP reactions at 70°C or, when combined with a thermostable reverse transcriptase, enables RT-LAMP at 70°C. Unit Definition One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 65°C. Enzyme Storage Buffer Composition 10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol, pH 7.5 @ 25°C. Storage Store at –25 to –15°C for 2 years. Avoid repeated freeze-thaw cycles. Notes 1. Keep the enzyme on ice or in an ice bath during use, and return it immediately to –20°C after use. 2. This product is for research use only. 3. For your safety and health, wear a lab coat and disposable gloves during handling. LAMP Experimental Example 1. Reaction Setup Component Volume (μL) Final Concentration 10× Hieff™ Super Bst DNA Polymerase Buffer 2.5 1× 100 mM MgSO₄ 0.75 3 mM + 2 mM in buffer = 5 mM dNTP Mix (25 mM each) 1.4 1.4 mM each dUTP (25 mM) (optional) 1.4 1.4 mM UDGase (1 U/μL) (optional) 1 0.04 U Template DNA 10 ng–1 μg — 10× Primers 2.5 — Hieff™ Super Bst DNA Polymerase (120 U/μL) 0.34–1* — ddH₂O to 25 — [Note]: The amount of Hieff™ Super Bst DNA Polymerase may be optimized according to specific experimental requirements. 10× Buffer composition: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄, 1% Tween-20, pH 8.8 @ 25°C. Mg²⁺ concentration can be adjusted between 4–10 mM depending on the assay. 10× Primers: 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B each. Our dNTP (Cat#10124), dUTP (Cat#10128), and UDGase (Cat#10303) are compatible with this product. 2. Reaction Conditions Temperature Time Purpose 25–37°C 5–10 min Degradation of uracil-containing templates (optional) 65–70°C 30–60 min Amplification 85°C 5 min Enzyme inactivation Documents: Safety Data Sheet 14411_MSDS_HB251205_EN.PDF Manuals 14411_Manual_Ver.EN20251203.pdf Show more