UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Features 10 U of this product was detected by E.coli 16S rDNA-specific TaqMan qPCR, and the result showed that E.coli genome residue was fewer than 10 copiesNo nucleic acid endonuclease, exonuclease and RNase residuesUracil is the sole base recognized by this enzyme Applications Remove ssDNA and dsDNA, but they are inactive to RNAEliminate false positive results due to contamination of PCR amplification products.Compatible with PCR, qPCR, RT-qPCR, etc Specifications Expression Host Recombinant E. coli with uracil DNA glycosylase gene Molecular Weight 24.8 kDa Purity ≥ 95%（SDS-PAGE） Heat Inactivation 95°C, 5~10 min Unit Definition One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C. Components Components No. Name 14455ES60 (100 U) 14455ES76 (500 U) 14455ES96 (10,000 U) 14455 Uracil DNA Glycosylase (UDG/UNG), 1 U/μL 100 μL 500 μL 10 mL Storage The product should be stored at -25℃ ~ -15℃ for two years. Figures Figure 1．UDG enzyme (0.025 U) fully digested 360 ng of 200 bp dU -DNA Electrophoresis results of 0.05 U, 0.025 U, 0.0125 U, 0.00625 U, 0.003125 U anti- contamination UDG enzyme with 360 ng of 200 bp dU -DNA incubated at 25°C for 30 min. Documents: Manuals 14455_Manual_HB230112_EN.pdf
