T4 DNA Polymerase catalyzes DNA synthesis in the 5'-3' direction in the presence of a template and primer. T4 DNA Polymerase has 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity. Compared to T4 DNA Polymerase (Cat#12901ES), UCF.METM T4 DNA Polymerase has lower host residue, making it suitable for applications that require stricter background control, such as pathogen detection. Applications Smoothing of DNA 5' or 3' overhanging ends Synthesis of labeled DNA probes via displacement reaction Synthesis of the second strand during site-directed mutagenesis Cloning of PCR products without reliance on ligation reactions Specifications Concentration 3 U/μL Unit definition One unit of activity is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotides (dNTPs) into a polynucleotide within 30 minutes at 37°C Components Components No. Name 14457ES64 14457ES77 14457-A UCF.METM T4 DNA Polymerase (3 U/μL) 50 μL 250 μL 14457-B 10 × Blue Buffer 100 μL 250 μL Shipping and Storage This product should be stored at -25 ~ -15℃ for 1 year. Figures Fig 1. Ultra-low host residue: 30 U < 3 copies Note: Detection of E. coli genomic residue in three batches of enzyme using qPCR method, with results showing < 3 copies per 30 U Documents: Safety Data Sheet 14457_MSDS_Ver.EN20250226.pdf Manuals 14457_Manual_Ver. EN20250226.pdf
