Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, retaining the DNA polymerase activity and 3'→5' exonuclease activity of DNA Polymerase I, but lacking the 5'→3' exonuclease activity. It can be used for random priming DNA labeling and the synthesis of the second strand of cDNA. Features No residual nucleases or RNases High amplification efficiency Applications DNA sequencing by the Sanger dideoxy method Second strand cDNA synthesis DNA labeling using random primers Specifications Source E. coli Molecular Weight 68.2 kDa Concentration 5 U/μL Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C Components Components No. Name 14458ES70 14458ES80 14458-A Klenow Fragment (5 U/μL) 40 μL 200 μL 14458-B 10×Klenow Fragment Buffer 1.25 mL 1.25 mL Shipping and Storage This product should be stored at -25 ~ -15℃ for 2 years. Figures Figure1. Detection of E. coli genomic DNA residue Note: The residual E. coli genomic DNA in three batches of Klenow Fragment (Cat#14458) is below 1 copy (at 50 U). Figure2. Product Stability Testing Note: The Klenow Fragment enzyme from Yeasen (Cat#14458), along with its supplier A, was subjected to a stability test at 37°C for durations of 7 and 14 days to measure the residual enzymatic activity. The findings indicate that Yeasen's Klenow Fragment maintained its activity without any significant decline after a week at 37°C, and retained approximately 90% of its initial activity after two weeks. Documents: Safety Data Sheet 14458_MSDS_Ver.EN20241210.pdf Manuals 14458_Manual_Ver.EN20241210.pdf
