Klenow (3'→5'exo-) DNA polymerase is a proteolytic product of E. coli DNA polymerase I, retaining the DNA polymerase activity of DNA polymerase I but losing the 5'→3' exonuclease activity. This enzyme has further removed the 3'→5' exonuclease activity through mutation. It can be used for DNA labeling with random priming and DNA sequencing by the dideoxy method, among other applications. Features No nickase or exonuclease residue Low residual host DNA High amplification efficiency Applications Random primer labeling Sanger dideoxy DNA sequencing Synthesis of the second strand of cDNA Synthesis of the second strand in mutagenesis reactions Specifications Source E. coli Molecular Weight 68.1 KDa Concentration 5 U/μL Unit Definition One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37°C Components Components No. Name 14460ES70 14460ES80 14460ES90 14460-A Klenow Fragment(3'→5' exo-) (5 U/μL) 40 μL 200 μL 1 mL 14460-B 10×Klenow Fragment (exo-) Buffer 250 μL 1.25 mL 5×1.25 mL Shipping and Storage This product should be stored at -25 ~ -15℃ for 2 years. Figures Figure1. Residual E.coli genomic DNA detection Note:The residual E. coli genomic DNA in three batches of Klenow Fragment (3'→5' exo-) (Cat#14460) is below 1 copy/50 U. Figure2. Product Stability Testing Note: Klenow Fragment (3'→5' exo-) from Yeasen and Supplier A was stored at 37°C for 7 and 14 days to assess residual enzyme activity. The results indicate that the residual activity of Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) after 14 days at 37°C is notably higher than that of the Supplier A. Figure3. NGS library yield Note: Using the same amount of Klenow Fragment (3'→5' exo-) (Cat#14460) from Yeasen and Supplier A for NGS library construction, the results show that the library yield with Yeasen 's Klenow Fragment (3'→5' exo-) (Cat#14460) at both 5 U and 10 U input is higher than that of Supplier A. Documents: Safety Data Sheet 14460_MSDS_Ver.EN20241210.pdf Manuals 14460_Manual_Ver.EN20241210.pdf
