Exonuclease I, sourced from a genetically engineered E. coli strain expressing the Exo I gene, exhibits exonuclease activity that digests single-stranded DNA from the 3' to 5' end. It sequentially releases deoxyribonucleotide 5'-monophosphates, preserving the integrity of the 5'-terminal dinucleotides. This enzyme is predominantly utilized for the breakdown and removal of primers following PCR amplification. It remains inactive towards double-stranded DNA and DNA strands with 3' hydroxyl ends that are blocked by phosphorylation or acetylation. Features No residual exonuclease (double-stranded), endonuclease, or RNase Inactivated by simply treating it at 80°C for 15 minutes Applications Remove single-stranded primers from the PCR reaction system before Sanger DNA sequencing or SNP analysis Remove single-stranded primers from the nested PCR reaction system Eliminate linear single-stranded DNA from the sample, leaving behind double-stranded DNA Specifications Source E. coli Molecular Weight 55 KDa Concentration 20 U/μL Unit Definition One unit is defined as the amount of enzyme required to catalyze the release of 10 nmol of acid-soluble nucleotides from single-stranded [3H]-DNA at a concentration of 0.17 mg/ml in a 50 μL reaction system containing 1X Exonuclease I reaction buffer at 37°C within 30 minutes Components Components No. Name 14535ES80 14535ES90 14535-A Exonuclease I (20 U/μL) 50 μL 250 μL 14535-B 10×Exonuclease I Reaction Buffer 1 mL 1 mL Shipping and Storage This product should be stored at -25 ~ -15℃ for 2 years. Figures Figure1. Digestion capability of Exonuclease I on single-stranded DNA Note: In a 20 μL reaction system, a final concentration of 15 pmol/μL single-stranded DNA substrate was added. Different amounts (0.63, 1.25, 2.5, 5, 10, 20 U) of Yeasen's and the Supplier A's Exonuclease I were used and incubated at 37°C for 15 minutes, followed by heat inactivation at 80°C for 15 minutes. Polyacrylamide gel electrophoresis was employed to detect the degradation of single-stranded DNA. The results demonstrated that Yeasen's Exonuclease I has the same ability to digest single-stranded DNA as Supplier A. Documents: Safety Data Sheet 14535_MSDS_Ver.EN20241210.pdf Manuals 14535_Manual_Ver.EN20241128
