Endonuclease VIII is a DNA repair enzyme that possesses both N-glycosylase activity and AP-lyase activity. The N-glycosylase activity can release damaged pyrimidine bases from double-stranded DNA, creating an abasic (AP) site. The AP-lyase activity then cleaves the phosphodiester bond at the AP site, resulting in a gap with a 5' phosphate and a 3' phosphate. The damaged bases that Endonuclease VIII recognizes and excises include: urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methoxyhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymidine, and methylhydroxypropanone. Features No residual of exonucleases, endonucleases, or RNases High-efficiency removal of damaged bases Applications Single-cell gel electrophoresis (Comet assay) Alkaline elution Alkaline unwinding Specifications Source E. coli Molecular Weight 29 KDa Concentration 10 U/μL Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 bp oligonucleotide duplex containing one AP site* in a 10 µL total reaction system with 10 pmol of fluorescently labeled oligonucleotide duplex in 1X Endonuclease VIII Reaction buffer at 37°C within 1 hour Inactivation Conditions Incubate at 75°C for 10 minutes Components Components No. Name 14536ES80 14536ES90 14536-A Endonuclease VIII(10 U/μL) 100 μL 500 μL 14536-B 10 × Endonuclease VIII Reaction Buffer 1.5 mL 1.5 mL Shipping and Storage The products should be stored at -25℃ ~ -15℃ for 1 year. Figures Figure1. Base excision repair effect comparison Note: 20 pmol of oligonucleotides were catalyzed by Endonuclease VIII from Yeasen and SupplierA, respectively, in a 20 µL reaction system with the addition of 5~40 U of Endonuclease VIII and equivalent enzyme activity of UDG (Cat#10303ES), and incubated at 37℃ for 1 hour. The results of agarose gel electrophoresis showed that the base excision effects of Yeasen 's Endonuclease VIII and Supplier A's were consistent. Documents: Safety Data Sheet 14536_MSDS_Ver.EN20241210.pdf Manuals 14536_Manual_Ver.EN20241210.pdf
