T7 Endonuclease I is encoded by gene 3 of bacteriophage T7. It recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday junctions (four-way DNA junctions), heteroduplex DNA, and also cleaves nicked double-stranded DNA at a slower rate. Cleavage occurs at the first, second, or third phosphodiester bond on the 5′ side of the mismatched base. Features High Cleavage Activity: 20 U of T7 Endonuclease I efficiently cuts 200 ng of mismatched dsDNA. High Protein Purity: ≥95% (as determined by SDS-PAGE). Specifications Cat NO. 14548ES72 / 14548ES86 Size 250 U / 2500 U Source Recombinant expression in Escherichia coli of gene 3 from bacteriophage T7 Concentration 10 U/μL Purity ≥95% Components Components No. Name 14548ES72 14548ES86 14548-A T7 Endonuclease I (10 U/μL) 25 μL 250 μL 14548-B 10× T7 Endonuclease I Reaction Buffer 1 mL 1 mL Storage This product should be stored at -25~-15℃ for 2 years. Application Detection of mutations following genome editing (e.g., CRISPR/Cas9 or TALEN-induced indels) Resolution of four-way DNA junctions or branched DNA structures Detection or cleavage of heteroduplex DNA and nicked DNA Random fragmentation of linear DNA for shotgun cloning Figures 1. High Cleavage Activity Figure 1. Verification of T7 Endonuclease I cleavage performance (Input: 200 ng DNA) Yeasen T7 Endonuclease I and imported Supplier A* T7 Endonuclease I were incubated with mismatched dsDNA substrates and analyzed by agarose gel electrophoresis. The results show that 20 U of Yeasen T7 Endonuclease I efficiently cleaves 200 ng of mismatched dsDNA, with a cleavage performance comparable to that of Supplier A*. Documents: Safety Data Sheet 14548_MSDS_HB251209 Manuals 14548_Manual_Ver.EN20251210
