T4 RNA Ligase 2, also known as T4 RNA ligase 2, is an ATP-dependent double-stranded RNA ligase that exhibits both inter- and intra molecular ligation activities on RNA strands. Compared to T4 RNA Ligase 1, this enzyme displays a significantly higher activity in ligating nicks in double-stranded RNA than in ligating single-stranded RNA ends. The proximity of a 5'-phosphate group and a 3'-hydroxyl group is required for ligation. Moreover, it has the unique capability to ligate between the 3´-hydroxyl group of RNA and the 5´-phosphate group of DNA within a double-stranded structure. Features Strong ligation activity: capable of effectively linking dsRNA substrates. No residual nuclease, nicking enzyme, or RNase. Applications Connecting the cleavage site of dsRNA. Connecting the 3´ hydroxyl group of RNA to the 5´ phosphate group of double-stranded DNA structure. Circular RNA preparation. Specifications Unit Definition One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37oC Source Recombinant protein expressed by E. coli is derived from the Y10A gene of T4 bacteriophage Purity ≥95% Reaction conditions React at 25oC for 1 hour Inactivation conditions React at 80oC for 5 min Components Components No. Name 14652ES80 14652ES90 14652-A T4 RNA ligase 2 (10 U/μL) 100 μL 500 μL 14652-B 10×T4 RL2 Reaction Buffer 500 μL 1.5 mL Shipping and Storage This product should be stored at -25 ~ -15oC for 1 years. Figures Figure 1. Verification of T4 RNA ligase 2 ligation efficiency. Note: The 20 μL reaction system contains dsRNA ligation substrates at a final concentration of 20 μM. Related Reading Synthesis of siRNA and the Role of T4 RNA Ligase 2 Documents: Safety Data Sheet 14652_MSDS_Ver.EN20241209 Manuals 14652_Manual_Ver.EN20250121
