UCF.ME™ High Affinity RNase Inhibitor (40 U/μL) is a recombinant RNase inhibitor expressed in E. coli. It specifically inhibits the activity of RNases A, B and C through binding noncovalently in a 1:1 ratio with high affinity. The host nucleic acid residue of UCF.ME™ High Affinity RNase Inhibitor (40 U/μL) is lower with the purification process specially developed by YEASEN, which is suitable for application with more stringent requirements on background bacteria, such as pathogen microorganism detection. Application Single-Cell Library Preparation RT-qPCR Specifications Source Recombinant E. coli with RNase Inhibitor gene Storage Buffer 20mM Hepes-KOH, 150mM KCl，8mM DTT，50%Glycerol，pH7.5±0.2@25℃ Unit Definition The required amount of RNase Inhibitor to inhibit 50% activity of 5-ng RNase A is defined as one unit. The activity of RNase A is measured by hydrolyzing of cyclic 2', 3'-CMP to generate 3'-CMP. Components Name 14675ES05 2 KU 14675ES20 20 KU 14675ES60 100 KU UCF.ME™ High Affinity RNase Inhibitor (40 U/μL) 50 μL 500 μL 2.5 mL Enzyme Storage Buffer Composition 20 mM Hepes-KOH, 150 mM KCl, 8 mM DTT, 0.5% NP40, 0.5% Tween-20, 50% Glycerol, pH 7.5 ± 0.2 25°C. Storage This product should be stored at -25~-15℃ for 2 years. Instructions 1. The recommended amount to add in a 20 μL system is 40 units (U), and the input quantity can be adjusted based on the actual results. 2. Incubate at 50℃ for 15 min, and inactivate the reaction by heating at 85℃ for 5 min. 3. Reverse transcription temperature: When used with 14604ES, 50℃ is recommended; for high GC content templates or complex templates, the reverse transcription temperature can be increased to 55℃-60℃. Notes 1. The product works in a wide pH range and exhibits maximal inhibitory activity at pH 7~8. 2. Please handle the product gently to avoid inactivation. 3. This product does not inhibit RNase H. 4. For your safety and health, please wear lab coats and disposable gloves for operation. Documents: Manual 14675_Manual_HB20260617
