Cas9 Nuclease is derived from the wild-type Streptococcus pyogenes and is an RNA-guided endonuclease that specifically cleaves double-stranded DNA (it can also cleave single-stranded DNA or RNA in the presence of a DNA PAM). The Cas9 cleavage site is located within the target sequence, three base pairs away from the PAM (NGG) region. Cas9 Nuclease has undergone codon optimization and design with a nuclear localization signal (NLS), and is expressed through recombinant expression in Escherichia coli. It exhibits high editing efficiency and can be used for gene modification in cells (such as hematopoietic stem cells, T cells, etc.), as well as for molecular diagnostics and pathogen detection. Features Optimized nuclear localization signal: The optimized nuclear localization signal (NLS) enhances editing efficiency. High editing efficiency: Consistently high editing efficiency both in vitro and in vivo. High purity: The purity is greater than or equal to 95%. High concentration: It is applicable under standard editing conditions and is also suitable for optimizing editing conditions in more challenging scenarios, such as in primary or embryonic cells, through microinjection or when conducting high-throughput screening of multiple gRNA sequences. Applications CRISPR/Cas gene editing Diagnostic and detection based on the CRISPR/Cas system Specifications Source The Cas9 gene from Streptococcus pyogenes is expressed through recombinant expression in Escherichia coli Molecular weight 163 KDa Concentration 10 mg/mL Purity ≥95% Tag His Endotoxin ≤10 EU/mg Components Components No. Name 14701ES60 14701ES76 14701ES03 14701-A Cas9 Nuclease（10 mg/mL） 10 μL 50 μL 100 μL 14701-B 10×Cas9 Nuclease Reaction Buffer 500 μL 1 mL 1 mL Shipping and Storage This product should be stored at -25 ~ -15oC for 1 years. Figures Figure 1. Results of Cas9 nuclease in vitro cleavage activity and gene knockout efficiency FAQ Q: When using 14701ES, how should one design single-guide RNA (sgRNA)? A: This product is derived from the wild-type Streptococcus pyogenes. The single-strand RNA (sgRNA) backbone can be used. 5’-NNNNNNNNNNNNNNNNNNNNGUUUUAGAGCUAUGCUGAAAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUG -3’ "N" represents a 20-nucleotide sequence that specifically binds to the DNA target sequence. We recommend purchasing the sgRNA synthesis kit with item number 11355ES from Yeasen. It has a high yield. Just design based on the target region, and the purified sgRNA product can be used in combination with 14701. Documents: Safety Data Sheet 14701_MSDS_Ver.EN20250120.pdf Manuals 14701_Manual_Ver.EN20241126.pdf
