E.coli Poly(A) polymerase, also known as Poly A tail-adding enzyme, catalyzes the addition of AMP converted from ATP to the 3 'end of single-stranded RNA in A template-independent manner to form Poly(A) tail. E.coli Poly(A) polymerase can use a variety of single-stranded RNA as a substrate, but DNA can not be used as a substrate. Double-stranded RNA and short oligonucleotides are not recommended as substrates. The Poly(A) tailing reaction catalyzed by E.coli Poly(A) Polymerase can only use ATP, but not ADP or dATP. The incorporation of CTP and UTP was less than 5% of that of ATP. When GTP is used, it cannot be added to the 3 'end of the RNA at all. Features When applied to miRNA detection, it demonstrates enhanced sensitivity. No residual nuclease or RNase. Applications RNA Modification. miRNA Expression Analysis. cDNA Synthesis & Reverse Transcriptases Products. RNA Synthesis In vitro Transcription (IVT). Specifications Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37oC Purity ≥90% Components Components No. Name 14801ES60 14801ES76 14801-A E.coli poly(A) Polymerase (5 U/μL） 20 μL 100 μL 14801-B 10×Reaction Buffer 100 μL 500 μL Shipping and Storage This product should be stored at -25 ~ -15oC for 2 years. Documents: Safety Data Sheet 14801_MSDS_Ver.EN20241209 Manuals 14801_Manual_Ver.EN20231009
