PBCV DNA Ligase, also known as SplintR ligase, PBCV-1 DNA Ligase, or Chlorella virus DNA ligase, is an ATP-dependent DNA ligase that efficiently catalyzes the ligation reaction of two adjacent DNA strands that are complementary to a single stranded RNA. In these two adjacent DNA strands, the one providing the 3' hydroxyl group is called the acceptor DNA strand, while the one providing the 5' phosphate group is called the donor DNA strand. During this ligation process, a complementary RNA strand acts as a "splint" or "scaffold" for the two DNA strands. The activity of PBCV DNA Ligase is much higher than that of traditional T4 DNA ligase and shows a strong affinity for DNA substrates with RNA as a splint (apparent Km = 1 nM, where a smaller Km value indicates a stronger affinity). It can detect unique RNA species at the subnanomolar level in complex mixtures, making it suitable for target enrichment processes in applications such as high-sensitivity RNA detection, next-generation sequencing, and molecular diagnostics. Features No residual nuclease, endonuclease, RNase, or phosphatase Applications Detection of microRNAs and other small RNAs based on probe method Connecting single-stranded DNA through "clamp" fixation by complementary RNA sequences Using DNA probes to achieve the detection of specific RNA Detection of SNP or Alternative Splicing RASL-seq analysis Specifications Source The DNA Ligase gene from PBCV-1 originating from Chlorella virus expressed in E.coli Molecular Weight 34 KD Concentration 25 U/µL Unit Definition One unit is defined as the enzyme amount required to achieve at least 50% completion of the ligation of 2 pmol FAM-labeled DNA:RNA hybrid substrate within 15 min at 25℃ in 1×PBCV Ligase buffer Inactivation Conditions 65℃, 20 min Components Component No. Name 14962ES80 14962ES90 14962-A PBCV DNA ligase (25 U/µL) 50 µL 200 µL 14962-B 10 x PBCV Ligase buffer 1 mL 1 mL Shipping and Storage The products should be stored at -25℃ ~ -15℃ for 2 years. Documents: Safety Data Sheet 14962_MSDS_Ver.EN20241210 Manuals 14962_Manual_Ver.CN20241210
