The FuniCutTM series of restriction endonucleases are rapidly acting, genetically engineered enzymes capable of precisely cutting DNA within 5 to 15 minutes. They are ideal for the rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. The FuniCutTM series shares a universal digestion buffer, simplifying the reaction setup, and offers excellent enzyme activity redundancy, making it easy to handle substrate excess or challenging templates. Specifications Enzyme Ncol Recognition Site 5'-C↓CATGG-3' 3'-GGTAC↑C-5' Recommended Reaction Conditions 1xFuniCutTM Buffer; optimal at 37℃ incubation Enzyme Activity 20 U/μL Inactivation Conditions Incubate at 80℃ for 20 minutes Isoschizomers Bsp191 Components Components No. Name 15018ES30 15018-A FuniCutTM Ncol 30 μL 15018-B 10 x FuniCutTM Buffer 1 mL 15018-C 10 x FuniCutTM Color Buffer* 1 mL [Note]: 10×FuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct loading onto agarose gels for electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel, while the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment. Shipping and Storage This product should be stored at -25℃~-15℃ for up to 2 years. Documents: Safety Data Sheet 15018_MSDS_HB250801_EN.PDF Manuals 15018_Manual_Ver.EN20250728.pdf