FuniCutTM enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCutTM enzymes show superior activity in the universal FuniCutTM Buffer and are able to digest DNA in 5~15 minutes.This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCutTM enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. Specifications Enzyme Xbal Cutting Site 5'-T↓CTAGA-3' 3'-AGATC↑T-5' Optimal Reaction Temperature 37℃ Sensitive to Heat Inactivation YES Type lls RE NO Methylation Sensitivity Not CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive,Not EcoKl Methylation-Sensitive, Not EcoBI Methylation-Sensitive Star Activity 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity Isoschizomers NO Components Components No. Name 15033ES76 15033-A FuniCutTM Xbal 500 μL 15033-B 10xFuniCutTM Buffer 3 x 1 mL 15033-C 10xFuniCutTM Color Buffer* 3 x 1 mL 【Note】: 10× FuniCutTM Color Buffer includes red and yellow tracking dyes, allowing direct loading of reaction products onto agarose gels without additional loading buffer. In 1% agarose gel electrophoresis, the red dye co-migrates with ~2500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to ~10 bp double-stranded DNA fragments. Shipping and Storage This product should be stored at -25℃ ~ -15℃ for up to 2 years. Documents: Safety Data Sheet 15033_MSDS_HB250801_EN.PDF Manuals 15033_Manual_Ver.EN20250801.pdf
