FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products. Features Rapid digestion: DNA was digested rapidly by enzyme for 5-15 min Universal buffer: one tube reaction, simplifying the process Stringent quality control Applications Molecular cloning Southern blotting SNP Southern blotting Genotyping Specifications Enzyme DpnI Cutting Site 5'-GAm6↓TC-3' 3'-CT↑Am6G-5' Optimal Reaction Temperature 37℃ Sensitive to Heat Inactivation YES Type IIs RE NO Methylation Sensitivity Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive Star Activity 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity Isoschizomers NO Components Components No. Name 15052ES50 (50 T) 15052-A FuniCut™ DpnI 50 μL 15052-B 10× FuniCut™ Buffer 1 mL 15052-C 10× FuniCut™ Color Buffer 1 mL Storage The product should be stored at -25℃ ~ -15℃ for two years. Please avoid repeated freeze-thaw. Documents: Manuals 15052_Manual_HB230113_EN.pdf
