FuniCut™ restriction enzymes are engineered via recombinant technology to enable precise DNA digestion within 5–15 minutes. They are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and other substrates. All FuniCut™ Fast Restriction Enzymes share a universal reaction buffer, simplifying the setup of digestion reactions. Additionally, these enzymes exhibit high activity redundancy, allowing efficient digestion even with excess substrate or challenging templates. Specifications Enzyme AvaII Recognition Site 5′-G↓GWCC-3′3′-CCWG↑G-5′ Recommended Reaction Conditions 1× FuniCut™ Buffer; incubate at 37°C. Enzyme Activity 10 U/μL Inactivation Conditions Incubate at 80°C for 20 minutes. Isoschizomers Bme18I, Eco47I Components Components No. Name 15091ES60(100 T) 15091-A FuniCut™ AvaII 100 μL 15091-B 10×FuniCut™ Buffer 1 mL 15091-C 10×FuniCut™ Color Buffer* 1 mL [Note]: *The 10× FuniCut™ Color Buffer contains red and yellow tracking dyes, enabling direct loading of digested samples onto agarose gels. In a 1% agarose gel, the red dye migrates similarly to a 2500 bp dsDNA fragment, while the yellow dye migrates similarly to a 10 bp dsDNA fragment.
