This product is a type II restriction endonuclease, a recombinant protein encoded by the BspQI gene from Bacillus sphaericus expressed in E. coli. It recognizes the sequence 5'-GCTCTTCN1/N4-3' and is mainly used for digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, obtaining specific sticky ends. Applications Digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends. Digesting DNA to obtain specific sticky ends. Specifications Recognition site 5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5' Concentration 10 U/µL Definition of Unit The amount of enzyme required to digest 1 µg of λDNA in a 50 µL reaction volume at 50°C within 1 hour is defined as one unit (U) of activity. Reaction conditions React at 50℃ for 60 min Components Name 15202ES76 15202ES86 15202ES92 BspQI (10 U/μL) 50 μL 250 μL 1 mL Shipping and Storage The products should be stored at -25℃ ~ -15℃ for 1 year. Documents: Safety Data Sheet 15202_MSDS_Ver.EN20250226.pdf Manuals 15202_Manual_Ver.EN20250226.pdf
