Nt.BstNBI is a nicking endonuclease that cleaves only one strand of double-stranded DNA (dsDNA) substrates, generating nicks without fully cutting the dsDNA. Specifications Cat NO. 15217ES84 Specification 2000 U Recognition Site 5'-GAGTCNNNN↓N-3'3'-CTCAGNNNNN-5' Enzyme Activity 10 U/μL Reaction Conditions 1× Nt.BstNBI Buffer; incubation at 55°C Inactivation Conditions Incubation at 80°C for 20 min Unit Definition One unit (U) is defined as the amount of enzyme required to completely convert 1 µg of supercoiled pUC19 DNA into open circular form in a 50 μL reaction volume within 1 hour at 55°C. Components Components No. Name 15217ES84(2000 U) 15217-A Nt.BstNBI (10 U/μL) 200 μL 15217-B 10×Nt.BstNBI Buffer 2×1 mL Shipping and Storage This product should be stored at -25℃~-15℃ for 2 years. Documents: Safety Data Sheet 15217_MSDS_HB251202_EN.PDF Manuals 15217_Manual_Ver.EN20251202.pdf
