The Quick Fuse Amplifier Reagent is a molecular biology reagent designed for the rapid, efficient, and specific isothermal amplification of nucleic acid fragments. Utilizing a combination of recombinase and polymerase, this reagent enables the swift amplification of target nucleic acids under a constant temperature range of 39–42°C. It is widely applied in biological research, clinical diagnostics, and molecular testing. This product is suitable for molecular diagnostic companies developing rapid, multiplex DNA detection kits. Product Components No. Name 16702ES16 16702ES50 16702-A Fuse Amplification Buffer 320 μL 1 mL 16702-B Fuse Amplification Enzyme Mix 1 108 μL 338 μL 16702-C Fuse Amplification Enzyme Mix 2（with exo III） 48 μL 150 μL 16702-D 200mM MgAC2 45 μL 140 μL *The Fuse Amplification Buffer is relatively viscous. When handling, aspirate slowly to ensure accurate volume measurement. It does not contain magnesium acetate. **The Fuse Amplification Enzyme Mix 2 (with exo III) already contains exo III. Storage Conditions Store at -25°C to -15°C. Shelf life: 1 year. Aliquot the reagent in advance to avoid repeated freeze-thaw cycles. Instructions for Use 1. Operation Steps (1) Reaction System Preparation: Add each component in the order listed in the table from top to bottom. No. Components Volume（μL） 100 μM primer F 0.2 100 μM primer R 0.2 50 μM probe 0.12 16702-B Fuse Amplification Enzyme Mix 1 6.75 16702-C Fuse Amplification Enzyme Mix 2 3 16702-A 2X Amplification buffer 20 DEPC H2O 1.93 Total 32.2 (2) Add 5 μL of template to the above system. (3) Thoroughly vortex and mix the reaction components, then add 2.8 μL of 200 mM magnesium acetate (16702-D) to the system to initiate the amplification reaction. Complete the subsequent steps as quickly as possible within 5 minutes. (4) Cap the PCR tube, vortex thoroughly to mix, and centrifuge briefly. Perform amplification according to the reference amplification protocol. 2. Reference Amplification Protocol Step Temp. Time Cycles 1 39℃* 1 min** 30 2 95℃ 2min 1 *The optimal reaction temperature can be selected between 39°C and 42°C depending on the specific system. **Fluorescence signal collection: Choose the appropriate channel based on the fluorescent label of the designed probe. Precautions 1. This product is intended for research use only. 2. For your safety and health, please wear a lab coat and disposable gloves during handling.
