The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a kit capable of performing isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve efficient, rapid, and highly specific detection reactions. It also supports room-temperature reaction system preparation. The buffers and enzyme components in the kit do not contain glycerol and can be lyophilized using the provided lyophilization protectant. Features Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products. High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors. Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction. Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup. Components Components No. Name 16730ES60 16730ES80 16730-A 4×Lyo LAMP Buffer 600 µL 6 mL 16730-B Bst Plus DNA Polymerase(Glycerol Free) 100 µL 1 mL 16730-C RT Enzyme mix(Glycerol Free) 50 µL 500 µL 16730-D 25×Fluorescent Dye 100 µL 1 mL 16730-E Lyophilization stabilizer 800 µL 8 mL Shipping and Storage This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light. Application Isothermal Amplification;Rapid detection of pathogenic bacteria. Figures 1. Workflow Figure 1. RT-LAMP Workflow. 2. Consistent performance between lyophilized and liquid formats. Figure 2. Comparable Detection Performance of Liquid and Lyophilized Formats. Both liquid and lyophilized versions of the RT-LAMP assay exhibit consistent sensitivity and specificity in detecting DNA and RNA targets, with similar amplification curves and reaction times. Documents: Safety Data Sheet 16730_MSDS_HB250713_EN.PDF Manuals 16730_Manual_Ver.EN20250708.pdf
