eSpCas9 mRNA encodes an engineered Streptococcus pyogenes Cas9 (eSpCas9) protein with enhanced expression specificity. When delivered into cells or in vivo for CRISPR-based gene and cell therapies, it expresses the S. pyogenes Cas9 nuclease with more than 10-fold reduction in off-target effects, while maintaining robust on-target editing efficiency. This mRNA features a Cap1 structure to enhance translation efficiency and protein expression levels, and includes a 100-nucleotide poly(A) tail that mimics mature eukaryotic mRNA, significantly improving its stability. Specifications Catalog Number 17105ES60/17105ES80 Format 100 μL /1mL Length 4619 nt Concentration 1 mg/mL Storage Buffer 1 mM Sodium citrate, pH6.5 Components Name 17105ES60 17105ES80 eSpCas9 mRNA 100μL 1mL Storage Store at –80°C. Valid for 2 years Notes 1. This product is for research use only. 2. For your safety and health, please wear a lab coat and disposable gloves when handling. 3. Upon receipt, immediately store the sample at –80°C. 4. During first use, wear disposable gloves, thaw the sample on ice, and use RNase-free reagents and consumables to avoid contamination. 5. After initial thawing, briefly centrifuge the sample gently and aliquot to avoid repeated freeze-thaw cycles. Documents: Safety Data Sheet 17105_MSDS_HB26120_EN.PDF Manuals 17105_Manual_Ver.EN20260120.pdf
