The SuperMethyl™-Fast Bisulfite Conversion Kit from Ellis Bio offers the revolutionary fastest and most efficient bisulfite conversion solution available for DNA methylation analysis. Featuring a newly engineered, ready-to-use fast bisulfite conversion reagent and a spin-column DNA purification method, this kit streamlines the workflow by combining conversion and purification in one seamless process. Simply add the SuperMethyl™-Fast Bisulfite Conversion Reagent to your sample and incubate for 6-8 minutes at 98 °C. Complete bisulfite conversion and DNA purification in under 35 minutes, this kit not only delivers speed but also precision, achieving over 99.5% conversion efficiency while minimizing DNA degradation. Its versatility makes it indispensable for both research and clinical applications. With an unmatched rapid workflow and exceptional performance, the SuperMethyl™-Fast Bisulfite Conversion Kit ensures reliable and reproducible results, making it the go-to choice for researchers who require both precision and quick turnaround times. Features Fastest Conversion Available:10X faster than leading industry kits Exceptional Performance:Excellent methylation protection; Compatible with a wide range of DNA inputs. Versatile Applications:Optimized for PCR, microarrays, endonuclease digestion, and next-generation sequencing (NGS). Cost-Effective:At just $179.99, competitively priced with a price-match guarantee. Components Component Volume and Ǫuantity SuperMethyl - Fast Conversion Reagent 1.5 mL x 7 vials SuperMethyl - Fast Binding Buffer 28 mL SuperMethyl - Fast Wash Buffer* 7 mL* SuperMethyl - Fast Desulphonation Buffer 11 mL SuperMethyl - Fast Elution Buffer 1.5 mL SuperMethyl - Spin Columns C Collection Tubes 50 pairs * SuperMethyl - Fast Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use. Shipping Conditions No Cold Chain Required; Shipped at Room Temperature Precautions / Handling Instructions Upon receipt of the kit, store the Conversion Reagent at 4°C. Workflow Figures 1. Figure 1 2. Figure 2. mESC gDNA converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. C-to-T conversion ratios were determined post-sequencing using unmethylated λ-DNA spike-in control. 3. Figure 3. mESC gDNA with 1% unmethylated lambda DNA spike-in were converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. False positive rates were calculated post-NGS as the percentage of CpG and non-CpG cytosines showing >1% unconverted C. 4.Versatile and robust methyl-seq performance Figure 4. Key performance metrics from whole-genome and targeted methylation profiling with the KAPA EvoPrep Kit and different conversion chemistries. Documents: Safety Data Sheet MSDS Manuals Manual Additional Resources: SuperMethyl™ Fast Data Sheet Microarray Data Brief
