The HieffTM Plasmid Mini Kit adopts HieffTM DNA Column P3 and a new solution system, and uses the conventional alkali cracking method to separate plasmid DNA. In the extraction process, toxic organic substances such as phenol and chloroform are not needed to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The HieffTM DNA Column P3 in the kit can specifically adsorb plasmid DNA, and does not adsorb protein, polysaccharide and other non-nucleic acid substances, so it is efficient, rapid and convenient. The extracted plasmid DNA has high purity and can be directly used in subsequent experiments such as DNA digestion, transformation and sequencing. Feature Wide Sample Range: Suitable for routine small-scale plasmid extraction; processes 2–6 mL of bacterial culture per prep. High DNA Purity: Free from protein and RNA contamination; DNA is ready for downstream applications such as digestion, transformation, and sequencing. High DNA Yield: Columns can bind up to 60 μg of plasmid DNA. Components Components No. Name 19001ES08 19001ES50 19001ES70 5 T 50 T 200 T Part I 19001-A RNase A (10 mg/mL) 15 µL 125 µL 500 µL Part II 19001-B RS Buffer P3* 1.5 mL 12.5 mL 50 mL 19001-C AC Buffer P3 500 µL 5 mL 20 mL 19001-D HieffTM DNA Column P3 5 50 200 19001-E 2 mL Collection Tube P3 5 50 200 19001-F Lysis Buffer(LB Buffer P3) 1.5 mL 12.5 mL 50 mL 19001-G Binding Buffer(BD Buffer P3) 1.8 mL 17.5 mL 70 mL 19001-H Deproteinization Buffer(PL Buffer P3*) 1.6 mL 16 mL 63 mL 19001-I Wash Buffer* 1.3 mL 13 mL 50 mL 19001-J Elution Buffer 1 mL 10 mL 20 mL Shipping and Storage Part I components are transported in ice bags and stored at -25~-15℃; Part II components are transported at room temperature and stored at room temperature. The product is valid for 12 months. Application Plasmid Extraction Figures 1. High Yields Figure 1. Comparison of Plasmid DNA Yields from Equal Volumes of Bacterial Culture Plasmid yield: 19001 > Supplier M > Supplier F. Documents: Safety Data Sheet 19001_MSDS_HB250626 Manuals 19001_Manual_Ver.EN20260127 Related Blog Plasmid Extraction Made Simple: From Mini to Maxi in One Solution — Pure, low-endotoxin plasmid DNA for cloning, transfection, and beyond
