The Hieff™ Gel Extraction Kit adopts Hieff™ DNA Column G1 and efficient sol system, which is suitable for recovering high-quality DNA fragments with the length of 100 bp-40 kb from TAE or TBE agarose gel. In the recovery process, toxic organic substances such as phenol and chloroform do not need to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The operation is simple, the sol time is short, the volume of added sol solution is small, and the whole purification process can be completed in 20 minutes. The Hieff™ DNA Column G1 in the kit can selectively adsorb up to 8 μg of DNA fragments, and does not adsorb enzymes, mineral oil and other impurities. The obtained DNA has high purity and can be directly used for subsequent experiments such as enzyme digestion, ligation, transformation, sequencing and library construction etc. Features Wide Application Range: Efficiently recovers DNA fragments from 50 bp to 15 kb. High Recovery Efficiency: Binds up to 8 μg of DNA per column. Fast & Efficient Workflow: Purify DNA in just 10 minutes with up to 85% recovery. Components Components No. Name 19101ES08 19101ES50 19101ES70 5 T 50 T 200 T 19101-A Hieff™ DNA Column G1 5 50 200 19101-B 2 mL Collection Tube G1 5 50 200 19101-C AC Buffer G1 0.5 mL 5 mL 20 mL 19101-D BD Buffer G1 2 mL 20 mL 80 mL 19101-E Wash Buffer* 1.3 mL 13 mL 50 mL 19101-F Elution Buffer 1 mL 10 mL 20 mL Figures Superior DNA Recovery Efficiency Figure 1. Agarose Gel Electrophoresis of Recovered DNA Fragments (0.5 kb & 1 kb) DNA fragments were recovered and analyzed via gel electrophoresis. Yeasen 19101 demonstrated greater band intensity and higher recovery efficiency compared to competitor T.
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