Salt Active UltraNuclease is a nonspecific endonuclease and has optimum activity at high salt concentration. Salt can play an important role in minimizing aggregation of protein or virus, which would be helpful for dissociation of DNA & RNA from proteins or other cellular components. In high salt conditions, enzymes are more likely to access the released DNA & RNA and degrade them. Nuclease which has high activity at high salt would be more effective to improve purification processes. The Salt Active UltraNuclease can be used to reduce the viscosity of cell supernatant and cell lysate, increase purification efficiency in high salt condition. The enzyme can also reduce host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. Features High Activity in High-Salt Conditions: Maintains optimal enzyme activity even at 500 mM salt concentration. Enhanced Viral Yield: High-salt tolerance reduces AAV particle aggregation, improving virus recovery. Reliable Product Quality: GMP-grade, animal-free, and antibiotic-free. Regulatory Support: DMF filing completed (MF037815). High Purity: ≥98% purity ensures consistent performance. Low Endotoxin: Suitable for sensitive applications. Compatible with ELISA Kits: Fully supported for downstream ELISA-based analysis. Specification Expression Host Recombinant, expressed in Escherichia coli Molecular Weight 24.7 kDa Isoelectric point 9.61 Purity ≥ 99% Enzyme activity 250-300 U/μL Specific activity ≥3×105 U/mg Protein purity (SEC-HPLC) ≥99% Bacterial endotoxins(gel-clot）
