This product consists of 9 highly purified and prestained proteins ranging in molecular weight from 25 kDa to 300 kDa (25,45,72,100,130,160,200,250,300 kDa), among them, 72 kDa is an orange band, 25 kDa is a green band. The labeled apparent molecular weight was calibrated by the molecular weight Marker of standard non-prestained proteins. Using this product, the state of protein electrophoresis and the effect of membrane transfer can be dynamically observed. After SDS-PAGE electrophoresis, the color bands were transferred to the o PVDF membrane and NC membrane. This product is conveniently packaged and is a ready-to-use product, do not heat, dilute or add reducing agents! After the prestained protein is combined with the dye, in different buffer systems, there is displacement. There are signs in the instructions, this product is only for reference when judging the molecular weight of the target protein. Features Bright color - three color predye, each strip average concentration of 2.5 μg Lower amount of this product into the gel hole - usually used for loading 5-10 μL each time Stable performance - 50℃ for 20 h, no degradation Long shelf life - can be stored at -30℃ ~ -15℃ for two years Applications Protein migration is monitored during SDS-PAGE electrophoresis Monitor protein transfer to the membrane after Western blotting Protein size analysis on SDS-PAGE and Western blotting Specifications Product Type Specs Protein Ladder Size Range 25 kDa to 300 kDa Number of Markers 9 Molecular Weight 25,45,72,100,130,160,200,250,300 kDa Stain Type 72 kDa is orange band, 25 kDa is green band, the rest are blue bands Storage Buffer 62.5 mM Tris-H3PO4 (pH 7.5), 2 mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 5 mM DTT, 0.02% (V/V) proclin300 Ready to Load ready-to-use product, do not heat, dilute or add reducing agents Components Components No. Name 20347ES76 20347ES90 20347 GoldBand™ Plus 3-color High Range Protein Marker (25-300 kDa) 2×250 μL 10×250 μL Storage The product shouldbe stored at -25℃ ~ -15℃ for two years. For regular use, it can be placed at 4℃, valid for three months. It is recommended to store in aliquots to avoid repeated freezing and thawing! Figures Figure 1 7% SDS-PAGE electrophoresis results Figure 2 Under different electrophoresis buffer conditions, the position of each band of this product Documents: Safety Data Sheet 20347_MSDS_HB220428_EN_1652931562544.PDF 20347_MSDS_HB220507_EN_1652931562497.PDF 20347_MSDS_HB220507_EN_1652931562808.PDF Manuals 20347_Manual_HB221103_EN.pdf Related Blog From Unstained to Tricolor: The Evolution of Protein Markers for Smarter Western Blotting
