Enterokinase Activity Assay Kit _ 20393ES

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Enterokinase (also known as enteropeptidase, EC 3.4.21.9) is a key enzyme that initiates protein digestion in the intestine. It precisely activates trypsinogen to trypsin, which th en cascade-activates chymotrypsinogen and procarboxypeptidase. Its expression is specifically localized in differentiated intestinal cells and goblet cells, making it both a core regulator of nutritional digestion and a reliable marker of intestinal epithelial maturation. The Enterokinase Activity Assay Kit is a rapid method for determining enterokinase activity in biological samples. This method detects enterokinase activity by cleaving a 7-amino-4-trifluoromethylcoumarin (AFC)-labeled peptide substrate containing an enterokinase recognition sequence. The intensity of the fluorescent product AFC (excitation wavelength Ex=380 nm, emission wavelength Em=500 nm) generated by the reaction is proportional to the enzyme activity in the sample. Product Information Component No. Component Name 20393ES60 (100 T) 20393-A 200× Enterokinase Substrate 24 μL 20393-B 1 mM AFC Standard 100 μL 20393-C Enterokinase Assay Buffer 60 mL Properties Cat# Component Name 20393ES60 (100T) 20393-A 200x Enterokinase Substrate 24 uL 20393-B 1 mM AFC Standard 100 uL 20393-C Enterokinase Assay Buffer 60 mL Application Figure 1. AFC standard curve. Figure 2. Sensitivity of the Yeasen Enterokinase (Cat#20395ES) detection kit. Data were collected at 25°C for 60 min according to the kit instructions. Storage Store at -25 to -15 degrees C. The unopened kit is valid for 1 year; after opening, valid for 6 months. Documents: Safety Data Sheet 20393_MSDS_HB260821 Manual 20393_Manual_HB20260819 Show more