PNGase F is an amidohydrolase that can cleave high-mannose, hybrid, and complex oligosaccharides linked by asparagine, specifically removing N-linked glycans. However, the conventional PNGase F enzymatic reaction often takes several hours to release antibody N-glycans. Due to the preferential release of glycans, incomplete deglycosylation might result in biased outcomes, with the obtained glycan distribution failing to represent the true composition of therapeutic antibodies. Therefore, rapidly obtaining an accurate N-glycan profile is crucial for glycosylation monitoring during the production of antibodies and antibody-fusion proteins used in biotherapeutics. Yeasen Fast PNGase F（Glycerol-free）is an optimized recombinant reagent capable of thoroughly and rapidly deglycosylating antibodies, immunoglobulins, fusion proteins, and other glycoproteins within minutes. This enzyme can swiftly and non-selectively remove all N-glycans, enabling direct downstream chromatography or mass spectrometry analysis. Yeasen Fast PNGase F（Glycerol-free） simplifies the experimental procedure while reducing experimental time without compromising sensitivity and reproducibility. Feature Fast—Complete and rapid deglycosylation in a few minutes. High purity—No protease, glycosidase contamination, purity ≥95%. Non-selective—Quickly and without preference remove all N-glycans. Good compatibility—Directly used for downstream chromatography or MS spectrometry analysis. Application Glycan Sequencing Glycoprotein Analysis Recombinant Glycoprotein Expression Specification English synonym Fast PNGase F（Glycerol-free） Source Yeast recombinant expression Storage buffer 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA Components Components No. Name 20406ES20 20406ES50 20406-A Fast PNGase F 20 μL 50 μL 20406-B Fast PNGase F Buffer（5×） 80 μL 200 μL Storage The product can be stored at 2 ~ 8℃ for one year, do not freeze. Figures Figure 1. Two-step enzymatic cleavage effect (protein substrate) 1: Protein Marker(Cat#20350ES) 2: Supplier N* + substrate 3: Supplier N* + substrate 4: Yeasen Fast PNGase F + substrate 5: Yeasen Fast PNGase + substrate F 6: Substrate 7: Yeasen Fast PNGase F 8: Supplier N* Figure 2. Two-step enzymatic cleavage effect (antibody) 1: Protein Marker(Cat#20350ES) 2: Supplier N* + antibody 3: Supplier N* + antibody 4: Yeasen Fast PNGase F + antibody 5: Yeasen Fast PNGase F + antibody 6: Antibody 7: Yeasen Fast PNGase F 8: Supplier N* Related Reading Fast PNGase F, Completing Deglycosylation in Just 10 Minutes Documents: Safety Data Sheet 20406_MSDS_HB260122 Manuals 20406_Manual_Ver. HB240524 Citations & References: 1. Wang N, Ji W, Jiao H, et al. A MERS-CoV-like mink coronavirus uses ACE2 as an entry receptor. Nature. 2025;642(8068):739-746. doi:10.1038/s41586-025-09007-w(IF:48.5)
