Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction. Properties English synonym Endo S Source E.coli recombinant expression Purity Detected by SDS-PAGE, purity > 95% Storage buffer PBS pH 7.5 Specific activity 8,000 U/mg Unit Definition The definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed. Components Components No. Name 20413ES80 20413ES90 20413-A Endo S 1000 U 5×1000 U 20413-B 10×Buffer 1 mL 5×1 mL Storage The product can be stored at -15~-25℃ for one year. Instructions Reaction systerm Components Volume IgG V Endo S 1 10×Buffer 1 H2O to 10 Note: After incubation at 37 °C for 1 hour, electrophoresis detection, load 2-3 μg of samples. The control group is the original protein. Experimental Example Electrophoresis of IgG under Endo S enzyme conditions Lane -: Control, without Endo S enzyme; Lane +: With Endo S enzyme. Notes 1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety！ 2. For research use only! Ver. EN20240426 Documents: Manuals 20413_Manual_HB240528_EN.pdf
