Endo H is a recombinant glycosidase that can cleave the core structure of high-mannose and some hybrid oligosaccharides in N-glycoproteins, removing the N-linked high-mannose from glycoproteins. Endo H is cloned from the corrugated actinomycete (Streptomyces plicatus) and recombinantly expressed in yeast. This product is tagged with His and is commonly used for complete deglycosylation of antibodies and related proteins. Feature High purity—No protease, glycosidase contamination, purity ≥95%. High Activity— 1,000,000 U/mL. Good compatibility—Can be used denaturing or no-denaturing conditions. Application Glycan Sequencing Proteomics Glycoprotein Analysis Recombinant Glycoprotein Expression Specification English synonym Endo H Source Yeast recombinant expression Molecular Weight 60 kDa Specific Activity 1,000,000 U/mL Storage buffer 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA Unit Definition One unit of endoglycosidase H is defined as the amount of enzyme required to hydrolyze >95% of the oligomannose from 10 μg of denatured RNase B in 1 hour at 37°C in a 10 μL reaction volume. Components Components No. Name 20414ES92 20414ES97 20414-A Endo H 10000 U 50000 U 20414-B1 Buffer 1 (10×) 100 μL 500 μL 20414-B2 Buffer 2 (10×) 200 μL 1000 μL Storage The product can be stored at -25 to -15℃ for one year. Documents: Manuals 20414_Manual_HB240528_EN.pdf
