Flag polypeptide is a hydrophilic polypeptide encoding 8 amino acids (DYKDDDDK). It can be used to detect specific anti-Flag monoclonal antibodies. In order to improve the sensitivity of detection, 3×Flag repeated polypeptide (3× flag-tag Peptide) has been developed. This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeated peptides have been widely used to purify Flag fusion proteins and competitively bind Anti-Flag monoclonal antibodies.The recommended working concentration of 3×Flag repeated polypeptide is 100-400 μg/mL. Features High stability High purity Short delivery Applications Purify Flag fusion proteins Competitively bind Anti-Flag monoclonal antibodies. Specifications Amino acid sequence H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH Molecular Formula C120H169N31O49S Molecular Weight 2861.87 Appearance White Powder Purity ≥98% Solubility Soluble in water (10mM) or 1% acetic acid Components Components No. Name 20571ES11 20571ES25 20571 3×Flag-tag Peptide 4 mg 25 mg Storage Unopened product is stable for 1 year at –25°C~-15°C.It is recommended to prepare fresh solutions as needed; storage as a stock solution is not advised. Documents: Manuals 20571_Manual_Ver.EN20251119.pdf
